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FLEXYTE® Assays Peptidylarginine Deiminases (PAD)
PAD Assay Principle
- Peptide substrate labelled with proprietary long lifetime 9-aminoacridine (9AA) fluorophore and incorporating an aromatic moiety which reduces the FLT of 9AA
- Deiminase catalysed citrullination of the arginine residue prevents cleavage by the protease, trypsin, thereby maintaining modulation of the FLT of 9AA
- Hence, deiminase activity is reported by a decrease in the measured FLT of the assay
PAD4 Assay
- A FLEXYTE® deiminase assay has been developed for PAD4, which is implicated in autoimmune diseases such as rheumatoid arthritis
- High assay sensitivity ensures low nM or pM enzyme requirements
- High Z'-factors confirm assay is suitable for HTS
- The protease protection approach to assaying deiminases has recently been extended to PAD2
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